Studying the expression of a lipase from Pyrococcus furiosus using response surfaces

► Expression of lipase Pf2001 in E. coli Rosetta(DE3)pLysS and BL21(DE3)pLysS. ► A factorial design 32 to verify the influence of temperature and IPTG on the expression. ► Higher lipase production when using the codon-rich strain Rosetta(DE3)pLysS. ► Optimal region of expression at 30 °C in 4 h, with a lipase production of 240 U/L. ► Production rate of the enzyme had effects on cell growth and activity.