Recombinant expression, purification and characterisation of the native glutamate racemase from Lactobacillus plantarum NC8

► First recombinant expression of the MurI from Lb. plantarum in E. coli. ► Use of a novel, l-Glu affinity chromatography for purification to single band. ► No use of His-tag or other affinity tags. ► First enzymological characterisation of MurI from Lb. plantarum in this detail.