Functional heterologous expression and purification of a mammalian methyl-CpG binding domain in suitable yield for DNA methylation profiling assays

► We improved heterologous expression of a mammalian methyl-CpG binding domain. ► Temperature at induction had a dramatic effect on soluble yield. ► A single-step purification yielded 11.3 mg/L (174 μg/L for existing procedures). ► This improved yield enables development of epigenetic profiling assays.