Purification, characterization and reconstitution into membranes of the oligomeric c-subunit ring of thermophilic FoF1-ATP synthase expressed in Escherichia coli

► The oligomeric c subunit ring was purified from the thermophilic FoF1-ATP synthase. ► A key in the purification was the introduction of a virtual His10-tag to the c-ring. ► The intactness of the isolated c-ring was confirmed by its homogeneity and activity. ► The isolated TFoc-rings were reconstituted into lipid membranes.