Sequencing, cloning and high-yield expression of a fungal β-N-acetylhexosaminidase in Pichia pastoris

► β-N-Acetylhexosaminidase from extremophilic fungus Talaromyces flavus was sequenced. ► Enzyme production significantly improved in P. pastoris. ► Single step purification of recombinant β-N-acetylhexosaminidase was sufficient. ► Wild type and recombinant enzyme retained the same biochemical properties. ► The first report on expression of fungal β-N-acetylhexosaminidase in yeast.