کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10870136 1073992 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Membrane protein synthesis in cell-free systems: From bio-mimetic systems to bio-membranes
ترجمه فارسی عنوان
سنتز پروتئین غشاء در سیستم های بدون سلول: از سیستم های بیومیدیزه به غشای زیستی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
چکیده انگلیسی
When taking up the gauntlet of studying membrane protein functionality, scientists are provided with a plethora of advantages, which can be exploited for the synthesis of these difficult-to-express proteins by utilizing cell-free protein synthesis systems. Due to their hydrophobicity, membrane proteins have exceptional demands regarding their environment to ensure correct functionality. Thus, the challenge is to find the appropriate hydrophobic support that facilitates proper membrane protein folding. So far, various modes of membrane protein synthesis have been presented. Here, we summarize current state-of-the-art methodologies of membrane protein synthesis in biomimetic-supported systems. The correct folding and functionality of membrane proteins depend in many cases on their integration into a lipid bilayer and subsequent posttranslational modification. We highlight cell-free systems utilizing the advantages of biological membranes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 17, 25 August 2014, Pages 2774-2781
نویسندگان
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