A neuronal transmembrane protein LRFN4 complexes with 14-3-3s and NCK1 to induce morphological change in monocytic cells via Rac1-mediated actin cytoskeleton reorganization

► LRFN4 signaling induces elongation of THP-1 via actin cytoskeleton reorganization. ►  LRFN4 complexes with 14-3-3 and NCK proteins to induce LRFN4-mediated elongation. ►  An SH3-2 domain of NCK1 interacts with the proline-rich region of LRFN4. ►  A 14-3-3 dimer interacts with both phospho-Ser 585 LRFN4 and phospho-Ser 85 NCK1. ►  A Rho family small GTPase, Rac1, is involved in LRFN4-mediated elongation.