کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10872239 | 1074111 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae
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کلمات کلیدی
GFPSACMitotic cell deathTerminal deoxynucleotidyl transferase-mediated dUTP nick-end-labelingAPC/C - APC / CROS - ROSanaphase-promoting complex/cyclosome - آنافاز تکاملی / cyclosomespindle assembly checkpoint - بازرسی مونتاژ اسپیندلTUNEL - تونلApoptosis - خزان یاختهایMitotic arrest - دستگیری متیوتیکBudding yeast - مخمر جوانه ایnocodazole - نوکودازولgreen fluorescent protein - پروتئین فلورسنت سبزPropidium iodide - پروتئین یدیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
We investigated the fate of budding yeast treated with nocodazole, a microtubule-depolymerizing drug. Cells died after mitotic arrest while staying in mitosis, suggesting that mitotic cell death, but not mitotic slippage, mainly occurs in nocodazole-treated cells. Nocodazole-treated cells showed features of apoptotic-like cell death, but not those of cell lysis or autophagy. Consistently, mitochondria-dependent production of reactive oxygen species was involved in the cell death. Similar cell death was also seen in cells after mitotic arrest by perturbation of the anaphase-promoting complex/cyclosome. In addition, caspase activity was found in nocodazole-treated cells, which was independent of the metacaspase, Mca1. Our results suggest that budding yeast can be a model to study mitotic cell death in cancer treatment with antimitotic drugs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 11, 3 June 2010, Pages 2387-2392
Journal: FEBS Letters - Volume 584, Issue 11, 3 June 2010, Pages 2387-2392
نویسندگان
Kingo Endo, Mayumi Mizuguchi, Aoi Harata, Go Itoh, Kozo Tanaka,