کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10872239 1074111 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae
چکیده انگلیسی
We investigated the fate of budding yeast treated with nocodazole, a microtubule-depolymerizing drug. Cells died after mitotic arrest while staying in mitosis, suggesting that mitotic cell death, but not mitotic slippage, mainly occurs in nocodazole-treated cells. Nocodazole-treated cells showed features of apoptotic-like cell death, but not those of cell lysis or autophagy. Consistently, mitochondria-dependent production of reactive oxygen species was involved in the cell death. Similar cell death was also seen in cells after mitotic arrest by perturbation of the anaphase-promoting complex/cyclosome. In addition, caspase activity was found in nocodazole-treated cells, which was independent of the metacaspase, Mca1. Our results suggest that budding yeast can be a model to study mitotic cell death in cancer treatment with antimitotic drugs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 11, 3 June 2010, Pages 2387-2392
نویسندگان
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