کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10872877 | 1074252 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Binding of JAB1/CSN5 to MIF is mediated by the MPN domain but is independent of the JAMM motif
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کلمات کلیدی
CSNYTHIVTCSN5Jab1NEDD8MALDI-TOFFCSMPNJamm - آرهIn vitro transcription/translation - رونویسی / ترجمه in vitromatrix-assisted laser desorption ionization-time of flight - زمان یونیزاسیون لیزر جذب لیزر ماتریس-زمان پروازfetal calf serum - سرم گوساله جنینCytokine - سیتوکینCOP9 signalosome - سیگنال COP9MIF - شهرMacrophage migration inhibitory factor - عامل مهارکننده مهاجرت ماکروفاژYeast two hybrid - مخمر دو مخلوطcalc - کالک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Binding of JAB1/CSN5 to MIF is mediated by the MPN domain but is independent of the JAMM motif Binding of JAB1/CSN5 to MIF is mediated by the MPN domain but is independent of the JAMM motif](/preview/png/10872877.png)
چکیده انگلیسی
Macrophage migration inhibitory factor (MIF) binds to c-Jun activation domain binding protein-1 (JAB1)/subunit 5 of COP9 signalosome (CSN5) and modulates cell signaling and the cell cycle through JAB1. The binding domain of JAB1 responsible for binding to MIF is unknown. We hypothesized that the conserved Mpr1p Pad1p N-terminal (MPN) domain of JAB1 may mediate binding to MIF. In fact, yeast two hybrid (YTH) and in vitro translation/coimmunoprecipitation (CoIP) analysis showed that a core MPN domain, which did not cover the functional JAB1/MPN/Mov34 metalloenzyme (JAMM) deneddylase sequence, binds to MIF comparable to full-length JAB1. YTH and pull-down analysis in conjunction with nanobead affinity matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry demonstrated that MIF(50-65) and MPN are sufficient to mediate MIF-JAB1 interaction, respectively. Finally, endogenous CoIP of MIF-CSN6 complexes from mammalian cells demonstrated that MPN is responsible for MIF-JAB1 binding in vivo, and, as CSN6 does not contain a functional JAMM motif, confirmed that the interaction does not require JAMM.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 7, 14 March 2005, Pages 1693-1701
Journal: FEBS Letters - Volume 579, Issue 7, 14 March 2005, Pages 1693-1701
نویسندگان
Anke Burger-Kentischer, Doris Finkelmeier, Michael Thiele, Jürgen Schmucker, Georg Geiger, Günter E.M. Tovar, Jürgen Bernhagen,