کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10877932 1076192 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of the retinoblastoma (Rb) gene and expression in response to environmental stressors in the intertidal copepod Tigriopus japonicus
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات علوم زمین و سیاره ای (عمومی)
پیش نمایش صفحه اول مقاله
Identification of the retinoblastoma (Rb) gene and expression in response to environmental stressors in the intertidal copepod Tigriopus japonicus
چکیده انگلیسی
There have been no reports thus far on the structure or molecular characterization of the retinoblastoma (Rb) gene of aquatic animals. Herein we describe the identification of the Rb gene of the intertidal copepod Tigriopus japonicus. In silico analyses revealed the conserved Rb domains of T. japonicus with those of protostomes. Phylogenetic analysis revealed that orthologs of Rb gene were evolved by an ancient split event in deuterostomes, while only a single Rb gene was conserved in protostomes except for Drosophila. The transcription of the T. japonicus Rb gene continuously increased across the molting transition from nauplius to the copepodid and adult stages, suggesting that it may play a developmental role in the molting process of T. japonicus. Information on Rb's response to environmental stressors, including toxin exposure, is lacking in copepods. To examine the transcriptional response to stressful conditions in laboratory culture conditions, copepods were exposed to UV-B radiation and different concentrations of metals, environmental toxins, and biocides. Transcription of the T. japonicus Rb gene was upregulated in response to about half of the 96 h-LD50 of UV-B radiation (12 kJ/m2) for 48 h, while the approximate 96 h-LD50 value (24 kJ/m2) of UV-B and relatively high concentrations of several toxins and biocides induced the downregulation of T. japonicus Rb mRNA expression. Taken together, our findings suggest that the T. japonicus Rb gene is sensitive to environmentally unfavorable conditions that can induce cell cycle alteration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Genomics - Volume 24, Part 3, December 2015, Pages 387-396
نویسندگان
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