Antibiotic susceptibility of Genistein and Alkaloids from Rhizophora apiculata

Antimicrobial prospective of dichloromethane and methanolic extracts of Rhizophora apiculata was to evaluate against urinary tract infective strains such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeurginosa, Proteus mirabilis and Klebsiella pneumoniae, methanolic extract showed better quality results at 250-350 µg/ml against all strains. And minimum inhibitory concentration and minimum bactericidal concentration were recorded at 160 µg/ml concentration level against for four strains excluding S. aureus at 240 µg/ml. Six phytochemicals (cardiac glycosides, saponins, tannins, coumarins, quinines and steroids) present in methanolic extract. Two spots were separated in thin layer chromatography and identified as genistein and unidentified alkaloids in addition to showed antibacterial activity in the TLC bioautography overlay assay screening. Together with characterized the genistein and alkaloids for FT-IR spectrum and displayed the occurrence of active functional groups such as hydrogen, carbonyl, carboxylic and ketones. These findings advise that the mangrove metabolites might be a potential candidate for the individualization of novel templates for the development of new antimicrobial agents or combinations of drugs for chemotherapy.