کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10889296 | 1080817 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Automation of the ELISpot assay for high-throughput detection of antigen-specific T-cell responses
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
IFN-γCEFPBMCsELISPOTCD8+ T-cells - CD8 + T-cellsHLA - آنتیژن گلبول سفید انسانیenzyme linked immunospot - آنزیم مرتبط با ایمنیAutomation - اتوماسیونinterferon - اینترفرونIFN - اینترفرون هاHigh-throughput - با توان بالاperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیCoefficient of Variation - ضریب تغییرHIV - ویروس نقص ایمنی انسانی human immunodeficiency virus - ویروس نقص ایمنی انسانی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Automation of the ELISpot assay for high-throughput detection of antigen-specific T-cell responses Automation of the ELISpot assay for high-throughput detection of antigen-specific T-cell responses](/preview/png/10889296.png)
چکیده انگلیسی
The enzyme linked immunospot (ELISpot) assay is a fundamental tool in cellular immunology, providing both quantitative and qualitative information on cellular cytokine responses to defined antigens. It enables the comprehensive screening of patient derived peripheral blood mononuclear cells to reveal the antigenic restriction of T-cell responses and is an emerging technique in clinical laboratory investigation of certain infectious diseases. As with all cellular-based assays, the final results of the assay are dependent on a number of technical variables that may impact precision if not highly standardised between operators. When studies that are large scale or using multiple antigens are set up manually, these assays may be labour intensive, have many manual handling steps, are subject to data and sample integrity failure and may show large inter-operator variability. Here we describe the successful automated performance of the interferon (IFN)-γ ELISpot assay from cell counting through to electronic capture of cytokine quantitation and present the results of a comparison between automated and manual performance of the ELISpot assay. The mean number of spot forming units enumerated by both methods for limiting dilutions of CMV, EBV and influenza (CEF)-derived peptides in six healthy individuals were highly correlated (r > 0.83, p < 0.05). The precision results from the automated system compared favourably with the manual ELISpot and further ensured electronic tracking, increased through-put and reduced turnaround time.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 344, Issue 1, 15 May 2009, Pages 1-5
Journal: Journal of Immunological Methods - Volume 344, Issue 1, 15 May 2009, Pages 1-5
نویسندگان
Coral-Ann M. Almeida, Steven G. Roberts, Rebecca Laird, Elizabeth McKinnon, Imran Ahmed, Katja Pfafferott, Joanne Turley, Niamh M. Keane, Andrew Lucas, Ben Rushton, Abha Chopra, Simon Mallal, Mina John,