کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10889339 | 1080826 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The ELISA Standard Save: Calculation of sample concentrations in assays with a failed standard curve
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
The enzyme-linked immunosorbent assay (ELISA) is often used to measure protein levels in plasma and other solutions. In order for the assay to be quantitative, a standard curve must be prepared for each assay. Technical blunders in preparing the standard curve can render otherwise representative sample values useless. In order to recover these data, a protocol has been developed whereby a new standard curve is generated using the ÎOD values of the samples. This new standard is applied to the original plate in order to determine sample concentrations. To validate this method, sample concentrations obtained using an acceptable standard curve were plotted against those obtained using the new standard curve. Linear regression analysis showed a 1:1 correlation between concentrations, with r2 values ranging from 0.98-0.99 and slopes ranging from 0.97-1.10. In this manner, data for these samples are preserved, saving the investigator the time and money involved in repeating experiments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 336, Issue 2, 31 July 2008, Pages 242-245
Journal: Journal of Immunological Methods - Volume 336, Issue 2, 31 July 2008, Pages 242-245
نویسندگان
Sudha Natarajan, Daniel G. Remick,