A novel single-step multiplex polymerase chain reaction assay for the detection of diarrheagenic Escherichia coli

► A novel mPCR has been developed to detect 10 target virulence genes of DEC strains. ► The method described will reduce the cost and improve the time efficiency of mPCR. ► The method performs simultaneous amplification of E. coli-virulence genes. ► The method would be a valuable contribution to routine diagnostic laboratory tests. ► The mPCR also provides important information for the physicians and others.