کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10893301 | 1082195 | 2005 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of stage of follicular growth during superovulation on developmental competence of bovine oocytes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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![عکس صفحه اول مقاله: Effect of stage of follicular growth during superovulation on developmental competence of bovine oocytes Effect of stage of follicular growth during superovulation on developmental competence of bovine oocytes](/preview/png/10893301.png)
چکیده انگلیسی
The final steps of oocyte capacitation and maturation are critical for embryonic development but detailed information is scarce on how the oocyte is affected during this period. In this study, 2033 oocytes were collected from 106 superovulated cattle at four different time points before ovulation. Follicular characteristics were measured and oocyte quality was assessed by morphology, mRNA expression of eight marker genes or developmental ability after in vitro/in vivo maturation and subsequent in vitro fertilization and culture. Approaching ovulation, expected increases in follicular size and cumulus expansion suggested progression of oocyte maturation. No differences were found in the expression patterns of analyzed genes, except for heat-shock-protein (Hsp) that was lower in in vivo matured oocytes collected shortly before ovulation. Oocytes collected at this time also had higher developmental ability measured as blastocyst rates (57.6%) after in vitro production while no differences were found between oocytes recovered earlier at the first three time points (39.3-41.5%). We conclude that oocytes recovered late in the preovulatory period are more developmentally competent than oocytes recovered at the pre-capacitation and the capacitation period, probably due to the former having matured in vivo. However, a precisely defined time for aspirating immature oocytes for subsequent in vitro development seems not to be crucial.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 63, Issue 4, 1 March 2005, Pages 1149-1166
Journal: Theriogenology - Volume 63, Issue 4, 1 March 2005, Pages 1149-1166
نویسندگان
P. Humblot, P. Holm, P. Lonergan, C. Wrenzycki, A.-S. Lequarré, C. Guyader Joly, D. Herrmann, A. Lopes, D. Rizos, H. Niemann, H. Callesen,