کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10913836 | 1088629 | 2013 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transdermal siRNA-TGFβ1-337 patch for hypertrophic scar treatment
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کلمات کلیدی
PVAECLDAPIDEPCRIPATGF-β2LAPERKHRPTGF-β3PVPqPCRTGFβ1DABWST-8HSCtransforming growth factor, beta 12-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt3,3′-diaminobenzidine - 3،3'-diaminobenzidine4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولH&E - H & EQuantitative PCR - PCR کمیSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNARadio immunoprecipitation assay - آزمایش ایمنی ادرار رادیوییAkt - آکتHypertrophic scar - اسکار هیپرتروفیIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیApoptosis - خزان یاختهایdiethylpyrocarbonate - دیاتیلپیر کربناتhematoxylin and eosin stain - رنگ آمیزی هماتوکسیلین و ائوزینnormal fibroblast - فیبروبلاست طبیعیWestern blotting - وسترن بلاتینگPET - پتHorseradish peroxidase - پراکسیداز هوررادیشprotein kinase B - پروتئین کیناز Bextracellular regulated protein kinases - پروتئین کیناز تنظیم شده خارج سلولیPropidium iodide - پروتئین یدیدpolyvinyl alcohol - پلی ونیل الکلpolyvinylpyrrolidone - پلی وینیل پیرولیدونlatency-associated peptide - پپتید وابسته به زمان تاخیرTransdermal patch - پچ ترشحی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hypertrophic scarring (HSc) is a fibroproliferative disorder of the dermis characterized by erythematous, swollen, and pruritic lesions of healing skin. An increased understanding of the role of TGFβ1 in the development of HSc provides the potential for treating HSc by down-regulating TGFβ1 expression. siRNAs that effectively interfered with TGFβ1 expression were screened. It was concluded that the siRNA-TGFβ1-337 was able to effectively down-regulate TGFβ1 expression in HSc fibroblasts. The effects of siRNA-TGFβ1-337 on cell proliferation, cell cycle, and apoptosis of HSc fibroblasts were investigated. It was shown that it inhibited cell proliferation, arrested cells in the G1 stage of the cell cycle, and induced apoptosis of HSc fibroblasts. The transdermal patch of siRNA-TGFβ1-337 was a combination of siRNA-TGFβ1-337 and a pressure-sensitive adhesive hydrogel. The treatment effects of the transdermal patch were assessed in an animal model established by transplanting human HSc to nude mice. Decreased expression of TGFβ1 was observed with treatment with the transdermal siRNA-TGFβ1-337 patch. Consequently, the treatment resulted in type I collagen down-regulation and regularly arranged scar fibroblasts being significantly reduced and undergoing apoptosis; the scar size was decreased significantly. Thus, our findings indicate that a transdermal siRNA-TGFβ1-337 patch is a potential treatment for hypertrophic scars.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 32, Issue 5, 24 June 2013, Pages 265-276
Journal: Matrix Biology - Volume 32, Issue 5, 24 June 2013, Pages 265-276
نویسندگان
Rui Zhao, Qitao Yan, Haili Huang, Jingye Lv, Wenli Ma,