کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10927772 | 1092883 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Freezing tolerance of sea urchin embryonic cells: Differentiation commitment and cytoskeletal disturbances in culture
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کلمات کلیدی
PBSPVPABSMe2SOCryoresistanceSeawater - آب دریاantibodies - آنتی بادی هاEthylene glycol - اتیلن گلیکولGene expression - بیان ژنTrehalose - ترهالوز Sea urchin - توتیای دریایی، خارپُشت دریاییRoom temperature - دمای اتاقphosphate buffered solution - محلول بافر فسفاتLiquid nitrogen - نیتروژن مایعCytoskeleton - چارچوب یاخته، سیتواسکلتون، اسکلت سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
This study focuses on the freezing tolerance of sea urchin embryonic cells. To significantly reduce the loss of physiological activity of these cells that occurs after cryopreservation and to study the effects of ultra-low temperatures on sea urchin embryonic cells, we tested the ability of the cells to differentiate into spiculogenic or pigment directions in culture, including an evaluation of the expression of some genes involved in pigment differentiation. A morphological analysis of cytoskeletal disturbances after freezing in a combination of penetrating (dimethyl sulfoxide and ethylene glycol) and non-penetrating (trehalose and polyvinylpyrrolidone) cryoprotectants revealed that the distribution pattern of filamentous actin and tubulin was similar to that in the control cultures. In contrast, very rare spreading cells and a small number of cells with filamentous actin and tubulin were detected after freezing in the presence of only non-penetrating cryoprotectants. The largest number of pigment cells was found in cultures frozen with trehalose or trehalose and dimethyl sulfoxide. The ability to induce the spicule formation was lost in the cells frozen only with non-penetrating cryoprotectants, while it was maximal in cultures frozen in a cryoprotective mixture containing both non-penetrating and penetrating cryoprotectants (particularly, when ethylene glycol was present). Using different markers for cell state assessment, an effective cryopreservation protocol for sea urchin cells was developed: three-step freezing with a low cooling rate (1-2 °C/min) and a combination of non-penetrating and penetrating cryoprotectants made it possible to obtain a high level of cell viability (up to 65-80%).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 71, Issue 1, August 2015, Pages 54-63
Journal: Cryobiology - Volume 71, Issue 1, August 2015, Pages 54-63
نویسندگان
Nelly A. Odintsova, Natalya V. Ageenko, Yulia O. Kipryushina, Mariia A. Maiorova, Andrey V. Boroda,