کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10927993 | 1092888 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biotransport and intracellular ice formation phenomena in freezing human embryonic kidney cells (HEK293T)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The objective of this study is to determine the cryobiological characteristics of human embryonic kidney (HEK293T) cells. The cell membrane hydraulic conductivity (Lpg) and the activation energy of water transport (ELp) were determined in the absence/presence of cryoprotectant agent (CPA), while the nucleation rate kinetic and thermodynamic parameters (ΩoSCN and κoSCN) were determined in the absence of CPA. Since dehydration and intracellular ice formation (IIF) are two factors that may cause damage to cells during the freezing process, systematical freezing experiments were carried out at different cooling rates (5, 10, 15, 20, 30, and 60 °C/min) under the commercial available cryomicroscopy (FDCS 196, Linkham, Waterfield, UK) to further explore the cryoinjury mechanism for HEK293T cells. By simultaneously fitting the water transport equation to the experimentally measured volumetric shrinkage data at 5, 10, and 15 °C/min, the “combined best fit” membrane permeability parameters for HEK293T cells in both phosphate buffer saline (PBS) and CPA media (0.75M Me2SO in PBS) are determined. They are Lpg = 2.85 Ã 10â14 m/s/Pa (0.17 μm/min/atm), ELp = 142.91 kJ/mol (34.13 kcal/mol) (R2 = 0.990), and Lpg[cpa] = 2.73 ± 0.44 Ã 10â14 m/s/Pa (0.16 ± 0.03 μm/min/atm), ELp[cpa] = 152.52 ± 27.69 kJ/mol (36.42 ± 6.61 kcal/mol) (R2 = 0.993), respectively. An optimal cooling rate Bopt (the highest cooling rate without IIF) was determined to be 14.24 °C/min in the absence of CPA. Additionally, the ice nucleation parameters (ΩoSCN and κoSCN) were averaged to be 1.31 ± 0.11 Ã 108 mâ2 sâ1 and 7.67 ± 2.55 Ã 109 K5 for the cooling rates 20, 30, and 60 °C/min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 68, Issue 2, April 2014, Pages 294-302
Journal: Cryobiology - Volume 68, Issue 2, April 2014, Pages 294-302
نویسندگان
Yunpeng Xu, Gang Zhao, Xiaoming Zhou, Weiping Ding, Zhiquan Shu, Dayong Gao,