The engrailed homeobox genes are required in multiple cell lineages to coordinate sequential formation of fissures and growth of the cerebellum

► Requirement for En1 and En2 in the cerebellum progenitor zone lineages was studied. ► En1/2 are required in ventricular zone-derived cells primarily for foliation. ► En1/2 are required in upper rhombic lip-derived cells primarily for growth. ► En2 alone is required mainly in progenitor cells for cerebellum growth and foliation. ► En1/2 together have multiples functions in growth and formation of fissures.