کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10933370 | 1093789 | 2009 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The evolutionarily conserved leprecan gene: Its regulation by Brachyury and its role in the developing Ciona notochord
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کلمات کلیدی
TBX6BrachyuryGFPNotochordGSTT-boxcis-regulationRFPEST - استAscidian - اسکیدینExpressed Sequence Tag - تکرار اظهار نظرBase pair(s) - جفت پایه (ها)CNS - دستگاه عصبی مرکزیcentral nervous system - سیستم عصبی مرکزیCiona - سیوناendoplasmic reticulum - شبکه آندوپلاسمی Muscle - عضلهanterior–posterior - قدامی-خلفیwild-type - نوع وحشیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازgreen fluorescent protein - پروتئین فلورسنت سبزred fluorescent protein - پروتئین فلورسنت قرمزglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In Ciona intestinalis, leprecan was identified as a target of the notochord-specific transcription factor Ciona Brachyury (Ci-Bra) (Takahashi, H., Hotta, K., Erives, A., Di Gregorio, A., Zeller, R.W., Levine, M., Satoh, N., 1999. Brachyury downstream notochord differentiation in the ascidian embryo. Genes Dev. 13, 1519-1523). By screening â¼Â 14 kb of the Ci-leprecan locus for cis-regulatory activity, we have identified a 581-bp minimal notochord-specific cis-regulatory module (CRM) whose activity depends upon T-box binding sites located at the 3â²-end of its sequence. These sites are specifically bound in vitro by a GST-Ci-Bra fusion protein, and mutations that abolish binding in vitro result in loss or decrease of regulatory activity in vivo. Serial deletions of the 581-bp notochord CRM revealed that this sequence is also able to direct expression in muscle cells through the same T-box sites that are utilized by Ci-Bra in the notochord, which are also bound in vitro by the muscle-specific T-box activators Ci-Tbx6b and Ci-Tbx6c. Additionally, we created plasmids aimed to interfere with the function of Ci-leprecan and categorized the resulting phenotypes, which consist of variable dislocations of notochord cells along the anterior-posterior axis. Together, these observations provide mechanistic insights generally applicable to T-box transcription factors and their target sequences, as well as a first set of clues on the function of Leprecan in early chordate development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 328, Issue 2, 15 April 2009, Pages 561-574
Journal: Developmental Biology - Volume 328, Issue 2, 15 April 2009, Pages 561-574
نویسندگان
Matthew P. Dunn, Anna Di Gregorio,