کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10934153 | 1093882 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genetic determinants of pancreatic ε-cell development
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recently, the expression of the peptide hormone ghrelin was detected in α-cells of the islets of Langerhans as well as in ε-cells, a newly discovered endocrine cell type, but it remains unclear how the latter is related in lineage to the four classical islet cell types, α-, β-, δ-, and PP-cells. Here, we provide further evidence that ghrelin is predominantly produced in the α-cells of mouse islets but also in single hormone ghrelin-secreting ε-cells. We additionally demonstrate that pancreatic ε-cells derive from Neurogenin3-expressing precursor cells and their genesis depends on Neurogenin3 activity. Furthermore, our data indicate that the number of ghrelin-producing cells is differentially regulated during pancreas morphogenesis by the homeodomain-containing transcription factors Arx, Pax4, and Pax6. Arx mutants lack ghrelin+ glucagon+ α-cells whereas Pax4 mutants develop an excess of these cells. Importantly, the ghrelin+ glucagonâ ε-cell population is not affected following Arx or Pax4 disruption. In contrast, the loss of Pax6 provokes an unexpected increase of the ghrelin+ glucagonâ ε-cell number which is not due to increased proliferation. Thus, we demonstrate that the development of ghrelin-producing cells is differentially dependent on Neurogenin3 in different domains of the gastrointestinal tract and that, in the endocrine pancreas, ε-cell genesis does not require Arx or Pax4 activities but is antagonized by Pax6.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 286, Issue 1, 1 October 2005, Pages 217-224
Journal: Developmental Biology - Volume 286, Issue 1, 1 October 2005, Pages 217-224
نویسندگان
R. Scott Heller, Marjorie Jenny, Patrick Collombat, Ahmed Mansouri, Catherine Tomasetto, Ole D. Madsen, Georg Mellitzer, Gerard Gradwohl, Palle Serup,