Understanding the mechanism of action of cell-penetrating antifungal peptides using the rationally designed hexapeptide PAF26 as a model

► The short hexapeptide PAF26 has been proposed as a model antifungal peptide. ► Here we review our latest understanding on the mechanism of action of PAF26. ► PAF26 has cell-penetrating properties that are linked to its antifungal activity. ► PAF26 action involves cell envelope interaction, internalization and cell killing.