Effects of cholesterol depletion on compartmentalized cAMP responses in adult cardiac myocytes

► Not all cAMP coupled receptors produce functional responses in adult cardiac myocytes. ► Functional responses correlate with receptors found in lipid rafts domains. ► Lipid raft receptors stimulate local cAMP production detected in PKA signaling domains. ► Non-lipid raft receptors produced cAMP detected outside of PKA signaling domains. ► Cholesterol depletion alters lipid raft dependent responses but not compartmentation.