کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956126 | 1098784 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA-224 is involved in the regulation of mouse cumulus expansion by targeting Ptx3
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کلمات کلیدی
PNDUTRsPTX3microRNA-224GCsCOCsmiRNAsEGFDMSO - DMSOECG - الکتروکاردیوگرام یا نوار قلبHCG - اچ سی جیEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاcorpus luteum - جسم زردDimethylsulfoxide - دیمتیل سولفواکسیدpostnatal day - روز پس از زایمانmicroRNAs - ریز آرانایgranulosa cells - سلولهای گرانولوزاCumulus-oocyte complexes - مجتمع های کومولوس-اووسیتUntranslated regions - مناطق غیر ترجمهpentraxin 3 - پنتراکسین 3Equine chorionic gonadotropin - گونادوتروپین کوریونی اسبhuman chorionic gonadotropin - گونادوتروپین کوریونی انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
MicroRNAs (miRNAs) are indicated to regulate ovarian development in a cell- or stage-specific manner. Our previous study showed that miR-224 is involved in TGF-β1-mediated follicular granulosa cell (GC) growth and estradiol (E2) production during the transition from the preantral to early antral stage by targeting Smad4. In this study, miR-224 was found to target pentraxin 3 (Ptx3), a gene critical for cumulus expansion during ovulation. In addition, PTX3 was up-regulated in mouse mural GCs and cumulus-oocyte complexes (COCs) by TGF-β1 treatment, which was partially mediated by miR-224. The effect of miR-224 during ovulation was further examined in vitro and in vivo by construction of an adenovirus-mediated expression vector for miR-224 (Ad-miR-224). In vitro studies demonstrated that miR-224 could perturb cumulus expansion in EGF-stimulated COCs by decreasing PTX3 secretion. In vivo studies also showed that injection of Ad-miR-224 into ovarian bursa decreased PTX3 expression and disrupted ovulation, which led to a decreased number of implantation sites and offspring being born. These results indicate that miR-224 may affect ovulation and subsequent embryo development by targeting Ptx3, suggesting potential roles for miRNAs in offering new treatments for ovulation disorder-associated infertility, or, conversely, designing new contraceptives.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 382, Issue 1, 25 January 2014, Pages 244-253
Journal: Molecular and Cellular Endocrinology - Volume 382, Issue 1, 25 January 2014, Pages 244-253
نویسندگان
Guidong Yao, Meng Liang, Ning Liang, Mianmian Yin, Mingrong Lü, Jie Lian, Yong Wang, Fei Sun,