mTOR inhibition with rapamycin causes impaired insulin signalling and glucose uptake in human subcutaneous and omental adipocytes

► Effects of rapamycin in human subcutaneous and omental adipocytes on glucose metabolism. ► Therapeutic rapamycin concentration reduces basal and insulin stimulated glucose uptake. ► Reduced glucose uptake is associated with impaired insulin signalling. ► Rapamycin effects on adipocytes may contribute to therapy associated diabetes.