کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956532 | 1099382 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Secreted phospholipase A2 group IIA is a neurotoxin released by stimulated human glial cells
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کلمات کلیدی
MAFPPBSLPSConAmAbFBSPLA2IFN-γConcanavalin ADMEM-F12DMSO - DMSOMTT - MTTAstrocytes - آستروسیتMonoclonal antibody - آنتی بادی مونوکلونالphospholipase A2 - آنزیم فسفولیپاز A2 Neuroinflammation - التهاب عصبیinterferon-gamma - اینترفرون گاماAlzheimer's disease - بیماری آلزایمرNeurodegeneration - تولید نوروژنیکDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH lipopolysaccharide - لیپوپلی ساکاریدmethyl arachidonyl fluorophosphonate - متیل آراکیدونیل فلوروفسفوناتNeuroprotection - محافظت نورونی یا محافظت از عصبPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMicroglia - میکروگلیاها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Secreted phospholipase A2 group IIA is a neurotoxin released by stimulated human glial cells Secreted phospholipase A2 group IIA is a neurotoxin released by stimulated human glial cells](/preview/png/10956532.png)
چکیده انگلیسی
Neuroinflammation, which is one of the hallmarks of neurodegenerative disorders such as Alzheimer's disease, involves secretion of pro-inflammatory mediators by activated glial cells. Secreted phospholipase A2 group IIA (sPLA2IIA) has been implicated as an inflammatory mediator contributing to various peripheral inflammatory conditions; however, little is known about the role this enzyme plays in neuroinflammation. Human microglia-like promonocytic THP-1 cells and human primary astrocytes were used to study sPLA2IIA expression, secretion and function. Production of sPLA2IIA by these cells was induced in response to stimulation by pro-inflammatory mediators at both mRNA and protein levels. Removal of sPLA2IIA from stimulated human microglia-like cell and astrocyte supernatants by immunosorbent caused significant reduction of their toxicity towards SH-SY5Y neuroblastoma cells. Both sPLA2IIA specific and non-specific PLA2 inhibitors exhibited no anti-cytotoxic or neuroprotective effects, suggesting that sPLA2IIA cytotoxicity is mediated by a non-enzymatic mechanism. The data obtained indicate that sPLA2IIA may contribute to the pathogenesis of neurodegenerative diseases involving neuroinflammation. Agents inhibiting the non-enzymatic actions of sPLA2IIA could be used to slow down progression of neurodegenerative processes that are driven by inflammation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 49, Issue 4, April 2012, Pages 430-438
Journal: Molecular and Cellular Neuroscience - Volume 49, Issue 4, April 2012, Pages 430-438
نویسندگان
Erika B. Villanueva, Jonathan P. Little, Gérard Lambeau, Andis Klegeris,