کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971602 | 1106483 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression regulation of zebrafish interferon regulatory factor 9 by promoter analysis
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کلمات کلیدی
IRFInterferon regulatory factor 9ISGF3ISGJanus kinase - کیناز جانوس STAT - آمارinterferon - اینترفرونinterferon stimulated response element - اینترفرون تحریک کننده عنصر پاسخIFN - اینترفرون هاgate - دروازهinterferon regulatory factor - عامل تنظیمی اینترفرونinterferon-stimulated gene factor 3 - عامل ژن تحریک کننده اینترفرون 3Signal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیExpression regulation - مقررات بیانJAK - چگونهGas - گازZebrafish - گورخرماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We previously showed that a fish interferon (IFN) regulatory factor 9 (IRF9) homologue, crucian carp Carassius auratus IRF9, displays constitutively nuclear localization and involvement in fish IFN-dependent JAK-STAT signaling; however, little is known about the expression regulation of fish IRF9. Here, we characterized the expression of zebrafish IRF9 by promoter analysis. Zebrafish IRF9 gene promoter contained several putative transcription factor binding sites, including one ISRE (IFN-stimulated response element), one GAS (IFN gamma activation sequence) and three GATEs (IFNγ activated transcriptional element, GATE1/2/3). Further sequence analyses revealed that GAS and GATE motifs existed in all promoters of IRF9 from mammals and fishes. Luciferase assays confirmed that zebrafish IRF9 promoter could be activated by zebrafish IFNÏs and zebrafish IFNγ2, as well as transcription factors IRF3, IRF7, and combination of IRF9 and STAT2. Treatment of recombinant crucian carp IFN protein or overexpression of zebrafish IFNγ2 both led to significant increase in crucian carp IRF9 mRNA and protein in cultured fish cells. Comparison of IFN-stimulated promoter activity revealed much more significant induction of zebrafish IRF9 by zebrafish IFNγ2 than by zebrafish IFNÏs. Mutation analyses showed that the putative GAS and GATE3 contributed to zebrafish IFNγ2-triggered IRF9 expression, whereas the putative ISRE and the other two GATEs were not functional for induction of zebrafish IRF9. These results together indicated that the expression property of IRF9 might be conserved from fish to mammals and that some not yet identified mechanisms could exist in IRF9 gene transcription regulation in response to IFNs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 41, Issue 4, December 2013, Pages 534-543
Journal: Developmental & Comparative Immunology - Volume 41, Issue 4, December 2013, Pages 534-543
نویسندگان
Jun Shi, Yi-Bing Zhang, Jian-She Zhang, Jian-Fang Gui,