کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971664 | 1106504 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cod cathelicidin: Isolation of the mature peptide, cleavage site characterisation and developmental expression
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کلمات کلیدی
DPHCFUTFAkiloDaltonkDaTrifluoroacetic acid - اسید TrifluoroaceticAtlantic cod - اقیانوس اطلسInnate immunity - ایمنی ذاتیFish immunity - ایمنی ماهیPeptide purification - تصفیه پپتیدmatrix-assisted laser desorption/ionization - جذب / یونیزاسیون لیزر ماتریس کمک می کندdays post hatch - روزه گرفتنMass spectrometry - طیف سنجی جرمیreversed phase - فاز معکوسMALDI - مالدیcolony forming units - واحدهای تشکیل دهنده کلنیAntimicrobial peptide - پپتیدهای ضدمیکروبیhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کارا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cathelicidin antimicrobial peptides are multifunctional peptides that are important in the innate immune system of mammals. Cathelicidins have been identified in several fish species. In this study we have isolated cathelicidin from Atlantic cod (Gadus morhua) and identified the cleavage site from the cathelin propart. This is the first isolation of a cathelicidin from teleost fish. The mature cathelicidin was found to be a 67-residues peptide, highly cationic with a pI of 13. Reversed phase chromatographic fractions containing the purified peptide had pronounced antimicrobial activity and the activity of the mature peptide was confirmed using a synthetic peptide. We examined the expression of cathelicidin during cod larvae early development using real-time PCR and detected expression that varied in the course of the first 68 days post hatching (dph). Two groups of larvae having a different food regime were compared. Cathelicidin expression was found to differ between the two groups and this could be linked to their food input. The presence and rapid adjustment of cathelicidin expression in the larvae indicate that the immune system of cod is active from early on in development and responds to external stimuli by the production of antimicrobial peptides.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 35, Issue 3, March 2011, Pages 296-303
Journal: Developmental & Comparative Immunology - Volume 35, Issue 3, March 2011, Pages 296-303
نویسندگان
Daniela C. Broekman, Dominik M. Frei, Gudjon A. Gylfason, Agnar Steinarsson, Hans Jörnvall, Birgitta Agerberth, Gudmundur H. Gudmundsson, Valerie H. Maier,