کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971897 | 1106748 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vaccination with outer membrane vesicles from Francisella noatunensis reduces development of francisellosis in a zebrafish model
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کلمات کلیدی
gDNACFUqPCRFrancisellaOMVsd.p.i.genomic DNA - DNA ژنومیQuantitative PCR - PCR کمیdays post infection - روز پس از عفونتOuter membrane vesicles - غشای بیرونی vesiclesgenome equivalents - معادل ژنومcolony forming units - واحدهای تشکیل دهنده کلنیVaccine - واکسنLive attenuated vaccines - واکسن های واکسن زندهLAV - کمZebrafish - گورخرماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Infection of fish with the facultative intracellular bacterium Francisella noatunensis remains an unresolved problem for aquaculture industry worldwide as it is difficult to vaccinate against without using live attenuated vaccines. Outer membrane vesicles (OMVs) are biological structures shed by Gram-negative bacteria in response to various environmental stimuli. OMVs have successfully been used to vaccinate against both intracellular and extracellular pathogens, due to an ability to stimulate innate, cell-mediated and humoral immune responses. We show by using atomic force and electron microscopy that the fish pathogenic bacterium F. noatunensis subspecies noatunensis (F.n.n.) shed OMVs both in vitro into culture medium and in vivo in a zebrafish infection model. The main protein constituents of the OMV are IglC, PdpD and PdpA, all known Francisella virulence factors, in addition to the outer membrane protein FopA and the chaperonin GroEL, as analyzed by mass spectrometry. The vesicles, when used as a vaccine, reduced proliferation of the bacterium and protected zebrafish when subsequently challenged with a high dose of F.n.n. without causing adverse effects for the host. Also granulomatous responses were reduced in F.n.n.-challenged zebrafish after OMV vaccination. Taken together, the data support the possible use of OMVs as vaccines against francisellosis in fish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 42, Issue 1, January 2015, Pages 50-57
Journal: Fish & Shellfish Immunology - Volume 42, Issue 1, January 2015, Pages 50-57
نویسندگان
Espen Brudal, Elisabeth O. Lampe, Léon Reubsaet, Norbert Roos, Ida K. Hegna, Ida Marie Thrane, Erling O. Koppang, Hanne C. Winther-Larsen,