کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971946 | 1106756 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of anti-inflammatory drugs in primary kidney cell culture of a freshwater fish
ترجمه فارسی عنوان
تأثیرات داروهای ضد التهابی در کشت سلولی اولیه کلیه یک ماهی آب شیرین
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
NSAIDSNF-kBiNOSL-NMMAFactor Nuclear kappa BNG-methyl-l-arginine3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideMTT - MTTEmerging contaminants - آلاینده های تازهNonsteroidal anti-inflammatory drugs - داروهای ضد التهابی غیر استروئیدیImmunosuppression - سرکوب سیستم ایمنیGenotoxicity - سمیت ژنتیکیinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییAnti-inflammatory - ضدالتهابNitric oxide - نیتریک اکسیدCell culture - کشت سلول
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
چکیده انگلیسی
The non-steroidal anti-inflammatory drugs are emerging contaminants in aquatic ecosystems. This study aimed to evaluate toxic effects of some representative drugs of this pharmaceutical group on primary culture of monocytic lineage of Hoplias malabaricus anterior kidney. The effects of diclofenac, acetaminophen and ibuprofen in cell viability, lipopolysaccharide (LPS)-induced NO production and genotoxicity were evaluated. Cytometry analysis CD11b+ cells showed 71.5% of stem cells, 19.5% of macrophages and 9% of monocytes. Cell viability was lower in the ficoll compared to percoll separation. LPS-induced NO production by these cells was blocked after treatment with dexamethasone and NG-Methyl-L-Arginine (L-NMMA). Exposure of the cells to diclofenac (0.2-200Â ng/mL), acetaminophen (0.025-250Â ng/mL) ibuprofen (10-1000Â ng/mL) reduced basal NO production and inhibited LPS-induced NO production at all concentrations after 24Â h of exposure. Genotoxicity occurred at the highest concentration of diclofenac and at the intermediary concentration of acetaminophen. Genotoxicity was also observed by ibuprofen. In summary, the pharmaceuticals influenced NO production and caused DNA damage in monocytic cells suggesting that these drugs can induce immunosuppression and genotoxicity in fish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 40, Issue 1, September 2014, Pages 296-303
Journal: Fish & Shellfish Immunology - Volume 40, Issue 1, September 2014, Pages 296-303
نویسندگان
João Luiz Coelho Ribas, Cesar A. da Silva, Lucas de Andrade, Gabrieli Limberger Galvan, Marta Margarete Cestari, Edvaldo S. Trindade, Aleksander R. Zampronio, Helena C. Silva de Assis,