NEMBASE4: The nematode transcriptome resource

► New anti-nematode drugs and vaccines are needed for animal and plant diseases. ► New targets for drug development and vaccine testing can be identified by comparative genomics. ► We have developed NEMBASE4, a database analysing 62 nematode species and 250,000 genes. ► NEMBASE4 can be used to test ideas of nematode gene function and identify new genes of interest. ► We demonstrate NEMBASE4 for analysing nematode drug targets and particularly horizontally transferred genes.