کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11001811 1051446 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Nucleolar localization signal and histone methylation reader function is required for SPIN1 to promote rRNA gene expression
چکیده انگلیسی
Spindlin1 (SPIN1), a histone modification reader protein, was enriched in the cell nucleolus and facilitated rRNA expression. However, how SPIN1 localizes to the nucleolus and its functional role in rRNA gene expression remain unresolved. Here, we identified a nucleolar localization signal in the N-terminal region of SPIN1 that is essential for its enrichment and function in the nucleolus. We also discovered that, in addition to its H3K4me3 recognizing activity, the H3R8me2a-recognizing capacity of SPIN1 is also indispensable for stimulating rRNA expression. Chromatin immunoprecipitation results indicated that SPIN1 is required for the association or assembly of selective factor 1 (SL1) complex, probably facilitating the initiation of rDNA transcription through its H3 K4me3-R8me2a reader function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 505, Issue 1, 20 October 2018, Pages 325-332
نویسندگان
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