کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11015598 | 1782694 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MicroRNA-613 promotes colon cancer cell proliferation, invasion and migration by targeting ATOH1
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The aim of the present study is to investigate the expression and function of miR-613 in colon cancer (CC) and illuminate the molecular mechanisms underlying miR-613-regulated CC progression. Our data demonstrated that miR-613 was upregulated in CC tissue samples (Pâ¯=â¯0.009) and human CC cell lines (HCT-116 and Lovo; Pâ¯=â¯0.001 and Pâ¯=â¯0.003, respectively), which also promoted the proliferation, invasion and migration of CC cells (Pâ¯<â¯0.05). The dual-luciferase reporter assay confirmed that Atonal homolog1 (ATOH1) was the target mRNA of miR-613. Rescue experiments showed that ATOH1 overexpression vector significantly reversed the stimulative effects of miR-613 mimic on the progression of HCT-116 and Lovo cells (Pâ¯<â¯0.001). Positive ATOH1 expression in CC tissues was significantly associated with lower grade (Ï2â¯=â¯3.592, Pâ¯=â¯0.043), lower TNM stage (Ï2â¯=â¯3.537, Pâ¯=â¯0.048) and better overall survival (P=0.041). Jun N-terminal kinase 1 (JNK1) pathway and Mucin 2 (MUC2) were the potential downstream proteins of miR-613/ATOH1. miR-613 is an oncogene in CC and promotes the proliferation, invasion and migration of CC cells by targeting ATOH1 likely via activating JNK1 pathway and upregulating MUC2.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 504, Issue 4, 12 October 2018, Pages 827-833
Journal: Biochemical and Biophysical Research Communications - Volume 504, Issue 4, 12 October 2018, Pages 827-833
نویسندگان
Xuanxuan Yang, Luo Zhang, Xing Song, Wenting He, Dachuan Zhang, Qicheng Lu, Jun Wu, Changping Wu, Jingting Jiang,