Core-shell red silica nanoparticles based immunochromatographic assay for detection of Escherichia coli O157:H7

An immunochromatographic strip based on core-shell red silica nanoparticles (core-shell red SiO2NPs) for detection of Escherichia coli O157:H7 (E. coli O157:H7) was developed. It was based on sandwich reaction between the capture antibody (test line), E. coli O157:H7 and core-shell red SiO2NPs labeled antibody. When there is E. coli O157:H7 in the sample, red band would appear both at test and control line. Otherwise, no color or reduced red color intensity at the test line compared with the positive sample.139