کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11025164 1701028 2019 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A screening test based on hematological and histological biomarkers to evaluate the environmental impacts in tambaqui (Colossoma macropomum) from a protected area in Maranhão, Brazilian Amazon
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
A screening test based on hematological and histological biomarkers to evaluate the environmental impacts in tambaqui (Colossoma macropomum) from a protected area in Maranhão, Brazilian Amazon
چکیده انگلیسی
Nowadays biomonitoring programs can benefit with mathematical models able to correlate biomarkers to monitor water pollution. The aim of this study was to develop a screening test based on hematological parameters and histological lesions in tambaqui (Colossoma macropomum), to allow the assessment of environmental impacts on fish inhabiting a protected area in Maranhão inside of Brazilian Amazon. Samples collected during three years (2012, 2013 and 2014) were grouped by season (dry and rainy) Water samples were also collected for physical chemistry analysis. Blood samples were stained with Acridine Orange to detect micronuclei and erythrocyte abnormalities. Gill tissues were stained with hematoxylin and counterstained with alcoholic eosin, and histopathological lesions were scored on a scale of 1-3, being 1 = minimal pathological importance, 2 = moderate pathological importance and 3 = marked pathological importance. A screening test for evaluating environmental impact was developed by fitting the measured data (necrosis, erythrocyte abnormalities, number of micronuclei) from tambaqui. A three-dimensional surface was fit to the empirical data. Our proposed model predicted the probability of necrosis (observed in euthanized animals) based on the numbers of micronuclei and abnormal erythrocytes (observed in blood samples from live animals) (correlation coefficient R = 0.89). The methodology could be applied for predicting contamination histories (chronic pollution that induces branchial lesions) in rivers using the micronucleus and erythrocyte abnormalities of the fishes (with a simple blood sample).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 214, January 2019, Pages 445-451
نویسندگان
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