The evaluation of Oryza sativa L (Black rice) extracts for detection of spermatozoa on the clothing and vaginal swab samples

Investigation of sexual assault cases from the evidence involving vaginal swab, clothing and others is examined by a forensic scientist. The explanation of trace findings on spermatozoa on clothing is often problematic due to the use of different staining methods. Conventional staining method used either Papanicolaou (PAP) or Dip quick® stain as synthetic dyes which are expensive imported material and harmful to human health. Therefore, the present study aims to determine the ability of Oryza sativa L (black rice) extract as a natural dye to detect spermatozoa on the clothing and vaginal swab casework samples for routine forensic examination. Results revealed that black rice extract has a highly effective for detecting spermatozoa on cloth and vaginal swab casework samples. There was no significantly different in the detection of spermatozoa compared with rapid PAP stain and Dip quick® stain. Results also showed that the staining of vaginal swab casework with black rice extracted can be used for PCR amplification of centromeric alphoid repeat gene on chromosome Y for 60 days. Moreover, the DNA extracted from stained semen slide generates a full profile of 16 alleles of STR typing. The results indicate that a new natural staining dye which extracted from black rice can be used to detect spermatozoa and identify a person from the trace evidence. The application of natural dyes for routine staining of spermatozoa from forensic specimens will decrease the expense to be spent in purchasing the synthetic dye and reduce their side effects on human and environment.