کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11029089 | 1646650 | 2018 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The fingerprint mapping and genotyping systems application on methicillin-resistant Staphylococcus aureus
ترجمه فارسی عنوان
کاربرد سیستم های اثر انگشت و سیستم ژنوتیپ در استافیلوکوک اورئوس مقاوم به متسییلین
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
چکیده انگلیسی
As a typical Gram-positive microorganism, S. aureus was recognized as common foodborne pathogenic bacteria in food industry. To study their individuality and pathogenicity mechanism, thirty-three Staphylococci strains were applied to the investigation with the identification of MRSA by PCR targeting on S. aureus specific 16S rRNA and femA genes as well as methicillin-resistant mecA and orfX elements by multiplex-PCR assay. Fingerprinting mapping was then employed using three typing systems (KZ/M13, IS256 and ERIC2) to genotype 33 MRSA strains. As the result indicated, all 33 Staphylococci strains were identified as MRSA. However, diversity occurred among different fingerprinting system results. KZ/M13 system and IS256 system both typed 10 genotypes while ERIC2 system had 8 genotypes. Based on the genotyping results, a discussion was performed in typing ability, discriminatory ability and accordance ratio. Given the above studies, a novel rapid detection method for MRSA was conducted with multiplex-PCR, which possessed rapidity and accuracy. Meanwhile, three fingerprinting systems showed high sensitivity, resolution and classification ratio in MRSA typing. These methods have a broad application prospect in food safety and epidemiology in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 125, December 2018, Pages 246-251
Journal: Microbial Pathogenesis - Volume 125, December 2018, Pages 246-251
نویسندگان
Jian Miao, Wenxin Wang, Wenyi Xu, Jianyu Su, Lin Li, Bing Li, Xia Zhang, Zhenbo Xu,