کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11031942 | 1645731 | 2019 | 43 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dietary optimal reduced glutathione improves innate immunity, oxidative stress resistance and detoxification function of grass carp (Ctenopharyngodon idella) against microcystin-LR
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present study reports on the effects of dietary glutathione (GSH) on non-specific immunity, oxidative stress resistance and detoxification in grass carp (Ctenopharyngodon idella) infected with microcystins. Six hundred and thirty juvenile grass carp with initial body weight of (5.10â¯Â±â¯0.09) g were randomly distributed into six groups with three replicates each and stocked in 18 tanks (35 fish per tank), fed six diets containing graded levels of GSH for 60â¯days. At the end of the feeding trial, ten fish per tank were selected and injected intraperitoneally with 200â¯Î¼g/kg body weight (BW) dose of pure microcystin-LR (MC-LR). The results showed that, at 0â¯h, 24â¯h of MC-LR injection, with the increase of dietary GSH content, serum lysozyme, alkaline phosphatase (ALP) and complement 3 (C3) levels, the activities and mRNA expression levels of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in liver, and liver GSH content increased first and then decreased (Pâ¯<â¯0.05). When dietary GSH content was 407.45â¯mg/kg diet, these parameters reached the highest levels (Pâ¯<â¯0.05), and in the same dose of GSH group, in addition to lysozyme activity at 24â¯h was significantly higher than that at 0â¯h, the other indicators at 24â¯h were significantly lower than those before exposure (at 0â¯h) (Pâ¯<â¯0.05). In contrast, serum cortisol and glucose, and liver reactive oxygen species (ROS) and malondialdehyde (MDA) contents decreased first and then increased (Pâ¯<â¯0.05), when dietary GSH content was 407.45â¯mg/kg diet, the above parameters reached the lowest levels (Pâ¯<â¯0.05), and in the same dose of GSH group their contents at 24â¯h were significantly higher than those before exposure (Pâ¯<â¯0.05). In addition, before MC-LR exposure, the activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) had no significant difference of all groups (Pâ¯>â¯0.05), liver glutathione S-transferase (GST) and its mRNA expression levels had a rising trend while liver glutathione reductase (GR) activities significantly decreased and then maintained a lower level with dietary GSH content increasing (Pâ¯<â¯0.05). At 24â¯h of MC-LR injection, serum ALT and AST activities decreased at first and then increased while liver GST and GR activities and their mRNA expression levels increased first and then decreased (Pâ¯<â¯0.05). Therefore, the basal diet supplemented with GSH 407.45â¯mg/kg could improve innate immunity, antioxidant stress ability and detoxification function of grass carp against MC-LR exposure. However, dietary excess GSH might promote oxidation, resulting in negative effect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 498, 1 January 2019, Pages 594-605
Journal: Aquaculture - Volume 498, 1 January 2019, Pages 594-605
نویسندگان
Jianhua Ming, Jinyun Ye, Yixiang Zhang, Xia Yang, Xianping Shao, Jun Qiang, Pao Xu,