Gelatins from Liza aurata skin: Structural characterization, in vitro and in vivo validation of acceleration epithelialization and cyto-protective effects

The resulting gelatins were structurally characterized by optical microscopy, differential scanning calorimetry (DSC), as well as by X-ray diffraction (XRD) and nuclearmagnetic resonance (NMR) spectroscopies. According to optical micrographs, DSC analysis, and XRD spectra, GGSGs are semi-crystalline polymers. Antioxidant activities of GGSGs were evaluated by five different methods in-vitro. GGSGs acted as a strong free-radical scavengers and they effectively protected skin genomic DNA against hydroxyl radical-induced oxidative damage. In addition, the wound healing efficiency of the GGSGs was assessed on circular excision wound rat model. GGSGs exhibited remarkable wound healing properties with 100% reduction of the wound area within 14 days. Histological examination also demonstrated a fully re-epithelialized wound with epidermal regeneration. These evidences depicted that GGSGs can be an ideal biomaterial for enhanced wound-healing effect presumably achieved through their antioxidant properties.