DNA purification using dynamic solid-phase extraction on a rotationally-driven polyethylene-terephthalate microdevice

• dSPE design on centrifugal PeT device with a unique mixing strategy was proposed.
• Increased fluidic control with novel adhesive tape valves on a PeT device.
• Multiplexed spin-dSPE device to run up to 4 samples simultaneously.
• Demonstrated strong singleplexed and multiplexed amplification following chip dSPE.

We report the development of a disposable polyester toner centrifugal device for semi-automated, dynamic solid phase DNA extraction (dSPE) from whole blood samples. The integration of a novel adhesive and hydrophobic valving with a simple and low cost microfabrication method allowed for sequential addition of reagents without the need for external equipment for fluid flow control. The spin-dSPE method yielded an average extraction efficiency of ∼45% from 0.6 μL of whole blood. The device performed single sample extractions or accommodate up to four samples for simultaneous DNA extraction, with PCR-readiness DNA confirmed by effective amplification of a β-globin gene. The purity of the DNA was challenged by a multiplex amplification with 16 targeted amplification sites. Successful multiplexed amplification could routinely be obtained using the purified DNA collected post an on-chip extraction, with the results comparable to those obtained with commercial DNA extraction methods. This proof-of-principle work represents a significant step towards a fully-automated low cost DNA extraction device.

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