کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1162925 1490919 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Preparation and evaluation of a novel molecularly imprinted polymer coating for selective extraction of indomethacin from biological samples by electrochemically controlled in-tube solid phase microextraction
ترجمه فارسی عنوان
تهیه و ارزیابی یک پوشش جدید پلیمری مولکولی جدید برای استخراج انتخابی ایندومتاسین از نمونه های بیولوژیکی با استفاده از الکتروشیمیایی کنترل می شود
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Molecularly imprinted polymer coated in-tube solid-phase microextraction was prepared.
• Electrochemically controlled in-tube solid-phase microextraction-HPLC was developed.
• Method was applied for selective extraction of indomethacin from biological samples.
• The effective parameters on the extraction efficiency of indomethacin were optimized.
• The method showed good linearity for indomethacin in the range of 0.1–200 μg L−1.

In the present work, an automated on-line electrochemically controlled in-tube solid-phase microextraction (EC-in-tube SPME) coupled with HPLC-UV was developed for the selective extraction and preconcentration of indomethacin as a model analyte in biological samples. Applying an electrical potential can improve the extraction efficiency and provide more convenient manipulation of different properties of the extraction system including selectivity, clean-up, rate, and efficiency. For more enhancement of the selectivity and applicability of this method, a novel molecularly imprinted polymer coated tube was prepared and applied for extraction of indomethacin. For this purpose, nanostructured copolymer coating consisting of polypyrrole doped with ethylene glycol dimethacrylate was prepared on the inner surface of a stainless-steel tube by electrochemical synthesis. The characteristics and application of the tubes were investigated. Electron microscopy provided a cross linked porous surface and the average thickness of the MIP coating was 45 μm. Compared with the non-imprinted polymer coated tubes, the special selectivity for indomethacin was discovered with the molecularly imprinted coated tube. Moreover, stable and reproducible responses were obtained without being considerably influenced by interferences commonly existing in biological samples. Under the optimal conditions, the limits of detection were in the range of 0.07–2.0 μg L−1 in different matrices. This method showed good linearity for indomethacin in the range of 0.1–200 μg L−1, with coefficients of determination better than 0.996. The inter- and intra-assay precisions (RSD%, n = 3) were respectively in the range of 3.5–8.4% and 2.3–7.6% at three concentration levels of 7, 70 and 150 μg L−1. The results showed that the proposed method can be successfully applied for selective analysis of indomethacin in biological samples.

An automated on-line method for selective extraction and preconcentration of the indomethacin in biological samples was developed.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 913, 24 March 2016, Pages 76–85
نویسندگان
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