کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1162940 1490915 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Solid-phase extraction based on ground methacrylate monolith modified with gold nanoparticles for isolation of proteins
ترجمه فارسی عنوان
استخراج فاز جامد بر اساس مونولیت متاکریلات زمینی اصلاح شده با نانوذرات طلا برای جداسازی پروتئین ها
کلمات کلیدی
نانوذرات طلا، گلیسیدیل متاکریلات مونولیت، پروتئین ها، استخراج فاز جامد
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• SPE sorbent with AuNPs immobilized onto the polymeric monolith was prepared.
• High recovery efficiency and satisfactory reusability of sorbent were demonstrated.
• Selective separation of proteins is easily achieved according to their pI values.

In this study, a novel polymeric material functionalized with gold nanoparticles (AuNPs) was prepared as solid-phase extraction (SPE) sorbent for isolation of proteins. The sorbent was synthesized from a powdered poly(glycidyl-co-ethylene dimethacrylate) monolith, and modified with ammonia, followed by immobilization of AuNPs on the pore surface of the material. To evaluate the performance of this SPE support, proteins were selected as test solutes, being the extraction conditions and other parameters (loading capacity and regenerative ability of sorbent) established. The results indicated that this sorbent could be employed to selectively capture proteins according to their pI, on the basis of the strong affinity of these biomacromolecules towards to AuNPs surface. The applicability of this sorbent was demonstrated by isolating protein species of interest (bovine serum albumin, cytochrome c and lectins in European mistletoe leaves), followed by SDS-PAGE analysis.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 917, 21 April 2016, Pages 37–43
نویسندگان
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