کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163170 1490927 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tunable nanogap devices for ultra-sensitive electrochemical impedance biosensing
ترجمه فارسی عنوان
دستگاه های نانولوله قابل تنظیم برای بی حس کننده امپدانس الکتروشیمیایی فوق العاده حساس
کلمات کلیدی
دستگاه های نانوذرات قابل تنظیم، استرپتاویدین، امپدانس الکتروشیمیایی، فوق العاده حساس، مقاومت انتقال الکترون
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• A tunable gold nanogap device was used as to electrochemical impedance biosensor.
• Linear range from 1 pM to 100 nM with LOD of 1 pM for streptavidin detection was obtained.
• The nanogap devices exhibit a satisfactory precision, stability, and reproducibility.
• The combination of electrochemical impedance technique and nanogap devices was achieved.

A wealth of research has been available discussing nanogap devices for detecting very small quantities of biomolecules by observing their electrical behavior generally performed in dry conditions. We report that a gold nanogapped electrode with tunable gap length for ultra-sensitive detection of streptavidin based on electrochemical impedance technique. The gold nanogap is fabricated using simple monolayer film deposition and in-situ growth of gold nanoparticles in a traditional interdigitated array (IDA) microelectrode. The electrochemical impedance biosensor with a 25-nm nanogap is found to be ultra-sensitive to the specific binding of streptavidin to biotin. The binding of the streptavidin hinder the electron transfer between two electrodes, resulting in a large increase in electron-transfer resistance (Ret) for operating the impedance. A linear relation between the relative Ret and the logarithmic value of streptavidin concentration is observed in the concentration range from 1 pM (picomolar) to 100 nM (nanomolar). The lowest detectable concentration actually measured reaches 1 pM. We believe that such an electrochemical impedance nanogap biosensor provides a useful approach towards biomolecular detection that could be extended to a number of other systems.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 905, 28 January 2016, Pages 58–65
نویسندگان
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