کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1163214 | 1490928 | 2016 | 7 صفحه PDF | دانلود رایگان |
• We reported a novel ECL-RET biosensor for sensitive analysis of casein kinase II activity.
• The successful ECL-RET between GQDs and GO could be established.
• GQDs was employed for casein kinase II activity monitoring and inhibition assay.
• Highly sensitive detection of CK2 activity and inhibition was achieved.
Herein, a novel electrochemiluminescence resonance energy transfer (ECL-RET) biosensor using graphene quantum dots (GQDs) as donor and graphene oxide (GO) as acceptor for monitoring the activity of protein kinase was presented for the first time. Anti-phosphoserine antibody conjugated graphene oxide (Ab-GO) nonocomposite could be captured onto the phosphorylated peptide/GQDs modified electrode surface through antibody–antigen interaction in the presence of casein kinase II (CK2) and adenosine 5′-triphosphate (ATP), resulting in ECL from the GQDs quenching by closely contacting GO. This ECL quenching degree was positively correlated with CK2 activity. Therefore, on the basis of ECL-RET between GQDs and GO, the activity of protein kinase can be detected sensitively. This biosensor can also be used for quantitative analysis CK2 activity in serum samples and qualitative screening kinase inhibition, indicating the potential application of the developed method in biochemical fundamental research and clinical diagnosis.
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Journal: Analytica Chimica Acta - Volume 904, 21 January 2016, Pages 58–64