کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163435 1490940 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid and sensitive detection of cholera toxin using gold nanoparticle-based simple colorimetric and dynamic light scattering assay
ترجمه فارسی عنوان
تشخیص سریع و حساس توکسین گوسفند با استفاده از روش ساده پراکندگی نور سنجی رنگی و پویا مبتنی بر نانوذرات طلا
کلمات کلیدی
توکسین کولورکتال، نانوذرات طلا، تست رنگ سنجی، آزمایش پراکندگی نور پویا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Gold nanoparticle based colorimetric and dynamic light scattering assay was developed for cholera toxin.
• The reported assay is sensitive enough to visually detect as low as 10 nM of cholera toxin.
• This assay is very fast and can specifically recognize cholera toxin over other diarrhetic toxins.

Herein, a rapid and simple gold nanoparticle based colorimetric and dynamic light scattering (DLS) assay for the sensitive detection of cholera toxin has been developed. The developed assay is based on the distance dependent properties of gold nanoparticles which cause aggregation of antibody-conjugated gold nanoparticles in the presence of cholera toxin resulting discernible color change. This aggregation induced color change caused a red shift in the plasmon band of nanoparticles which was measured by UV–Vis spectroscopy. In addition, we employed DLS assay to monitor the extent of aggregation in the presence of different concentration of cholera toxin. Our assay can visually detect as low as 10 nM of cholera toxin which is lower than the previously reported colorimetric methods. The reported assay is very fast and showed an excellent specificity against other diarrhetic toxins. Moreover, we have demonstrated the feasibility of our method for cholera toxin detection in local lake water.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 892, 10 September 2015, Pages 167–174
نویسندگان
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