کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163453 1490972 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Zone fluidics for measurement of octanol–water partition coefficient of drugs
ترجمه فارسی عنوان
سیگنال های منطقه برای اندازه گیری ضریب پراکندگی آب اکتانولا
کلمات کلیدی
منطقه سیویلیک، ضریب پارتیشن آب اکتانول، کشف مواد مخدر
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• An automated zone-microfluidic system is presented for screening of octanol–water partition coefficient (Pow).
• Requires only one-phase measurement.
• Vertically aligned holding column improves eliminates zone breakage.

A novel zone fluidics (ZF) system for the determination of the octanol–water partition coefficient (Pow) of drugs was developed. The ZF system consisted of a syringe pump with a selection valve, a holding column, a silica capillary flow-cell and an in-line spectrophotometer. Exact microliter volumes of solvents (octanol and phosphate buffer saline) and a solution of the drug, sandwiched between air segments, were sequentially loaded into the vertically aligned holding column. Distribution of the drug between the aqueous and octanol phases occurred by the oscillation movement of the syringe pump piston. Phase separation occurred due to the difference in densities. The liquid zones were then pushed into the detection flow cell. In this method, absorbance measurements in only one of the phase (octanol or aqueous) were employed, which together with the volumes of the solvents and pure drug sample, allowed the calculation of the Pow. The developed system was applied to the determination of the Pow of some common drugs. The log (Pow) values agreed well with a batch method (R2 = 0.999) and literature (R2 = 0.997). Standard deviations for intra- and inter-day analyses were both less than 0.1log unit. This ZF system provides a robust and automated method for screening of Pow values in the drug discovery process.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 860, 20 February 2015, Pages 1–7
نویسندگان
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