A novel type of matrix for surface-assisted laser desorption–ionization mass spectrometric detection of biomolecules using metal-organic frameworks

• This is the first report on the SALDI-MS analysis of polar carbohydrates using MOF as matrix.
• 3D MOF for SALDI-MS matrix was developed for biomolecule analysis without chemical modification.
• Good signal reproducibility and low background interferences were achieved.
• MOF for SALDI-MS can be used for quantitative and qualitative analysis of polar carbohydrate molecules.

A 3D metal-organic framework (MOF) nanomaterial as matrix for surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) and tandem mass spectrometry (MS/MS) was developed for the analysis of complex biomolecules. Unlike other nanoparticle matrices, this MOF nanomaterial does not need chemical modification prior to use. An exceptional signal reproducibility as well as very low background interferences in analyzing mono-/di-saccharides, peptides and complex starch digests demonstrate its high potential for biomolecule assays, especially for small molecules.

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