کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1163868 1490982 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ag nanoclusters as probes for turn-on fluorescence recognition of TpG dinucleotide with a high selectivity
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Ag nanoclusters as probes for turn-on fluorescence recognition of TpG dinucleotide with a high selectivity
چکیده انگلیسی


• Fluorescent Ag NCs can be grown at the DNA bulge site for dinucleotide identification.
• The growth of Ag NCs is highly DNA sequence dependent.
• Discrimination of TpG dinucleotide from the other dinucleotides can be achieved with a high selectivity.

CpG dinucleotide in DNA has a great tendency to mutate to TpG dinucleotide and this transition can cause some serious diseases. In this work, fluorescent Ag nanoclusters (Ag NCs) were employed as useful inorganic fluorophores for the potential of selectively discriminating TpG dinucleotide from CpG dinucleotide. Opposite the base Y of interest in YpG dinucleotide (Y = C or T), a bulge site was introduced so as to make the base Y to be unpaired and ready for Ag+ binding. Such that the unpaired Y and context base pairs can provide a specific space suitable for creating fluorescent Ag NCs. We found that in comparison with CpG dinucleotide, TpG dinucleotide is much more efficient in growing fluorescent Ag NCs. Therefore, mutation of CpG dinucleotide to TpG can be identified by a turn-on fluorescence response and a high selectivity. More interestingly, Ag NCs exhibit a better performance in the TpG recognition over the other dinucleotides (Y = A and G) than the previously used organic fluorophores. Additionally, the effectiveness of the bulge site design in discriminating these dinucleotides was evidenced by control DNAs having the abasic site structure. We expect that a practical method for TpG dinucleotide recognition with a high selectivity can be developed using the bulge site-grown fluorescent Ag NCs as novel probes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 850, 19 November 2014, Pages 78–84
نویسندگان
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