کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1164085 1490974 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of gold nanoparticle based colorimetric method for quantitatively studying the inhibitors of Cu2+/Zn2+ induced β-amyloid peptide assembly
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development of gold nanoparticle based colorimetric method for quantitatively studying the inhibitors of Cu2+/Zn2+ induced β-amyloid peptide assembly
چکیده انگلیسی


• Quantitatively study the inhibitors of Cu2+/Zn2+ induced β-amyloid aggregation.
• The half maximal inhibitory concentrations (IC50) of inhibitors were obtained.
• This method simplifies and reduces the cost of the inhibitors screening procedure.
• This high throughput technology is helpful for anti-dementia drugs discovery.

In this paper, a kind of gold nanoparticle (GNP)-based colorimetric assay has been developed for studying the reversible interaction of β-amyloid peptide (Aβ) with Cu2+ and Zn2+, and quantitatively analyzing four inhibitors (i.e., EDTA, EGTA, histidine and clioquinol) of Cu2+/Zn2+ induced Aβ assembly. The inhibition efficiencies (e.g., half maximal inhibitory concentration, IC50 value) of these inhibitors could be measured in this work. As far as we know, these IC50 values were reported at the first time. In this assay, the streptavidin conjugated GNPs (SA-GNPs) were employed as indicators to monitor the Cu2+/Zn2+ induced aggregating/disaggregating behaviors of biotin modified β-amyloid 1–16 peptides (Aβ1–16(biotin)). Because of high affinity of streptavidin (SA) with biotin, the aggregating/disaggregating of Aβ1–16(biotin) results in the significant color change of SA-GNPs. Furthermore, we demonstrate that the assay can be used as an effective tool for designing anti-dementia drugs through quantitative analysis of the interactions of four representative inhibitors with Cu2+/Zn2+ induced Aβ assembly.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 858, 9 February 2015, Pages 42–48
نویسندگان
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