کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1164129 | 1491020 | 2014 | 8 صفحه PDF | دانلود رایگان |
• We constructed a modified electrode for detecting uric acid contained in human serum.
• Electrochemical detection of uric acid was comparable with the enzymatic method.
• Interference of ascorbic acid was not observed.
Glassy carbon electrodes (GCE) were sequentially modified by cysteamine-capped gold nanoparticles (AuNp@cysteamine) and PAMAM dendrimers generation 4.5 bearing 128-COOH peripheral groups (GCE/AuNp@cysteamine/PAMAM), in order to explore their capabilities as electrochemical detectors of uric acid (UA) in human serum samples at pH 2. The results showed that concentrations of UA detected by cyclic voltammetry with GCE/AuNp@cysteamine/PAMAM were comparable (deviation <±10%; limits of detection (LOD) and quantification (LOQ) were 1.7 × 10−4 and 5.8 × 10−4 mg dL−1, respectively) to those concentrations obtained using the uricase-based enzymatic-colorimetric method. It was also observed that the presence of dendrimers in the GCE/AuNp@cysteamine/PAMAM system minimizes ascorbic acid (AA) interference during UA oxidation, thus improving the electrocatalytic activity of the gold nanoparticles.
Figure optionsDownload as PowerPoint slide
Journal: Analytica Chimica Acta - Volume 812, 17 February 2014, Pages 18–25